Part:BBa_K4392011:Design

exoB2 gene from Azospirillum brasilense

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 629
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 37
  Illegal NgoMIV site found at 526
  Illegal NgoMIV site found at 723
  Illegal NgoMIV site found at 850
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 406
  

Design Notes

FP: TATATCTAGAATGAACACGCCCCACACCGT (Tm=64.5 C; %GC=47) RP: TATATCTAGATCACTCGGCGGCCTGCGCCA (Tm=65.4 C; %GC=53)

Source

Derived from the genome of A. brasilense